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    Protein Simple Inc sally sue proteinsimple
    Sally Sue Proteinsimple, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 94/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sally+sue+proteinsimple/Sally+Sue/pmc11980838__sciadv__adq5484_sm-47-31-33
    Average 94 stars, based on 70 article reviews
    sally sue proteinsimple - by Bioz Stars, 2026-09
    94/100 stars

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    Article Title: Lower Tubulin Expression in Glioblastoma Stem Cells Attenuates Efficacy of Microtubule-Targeting Agents.
    Article Snippet: Antibody Supplier Identifier Total α-tubulin Cell Signaling Technology Cat# 2144, RRID: AB_2210548 Total β-tubulin Abcam Cat# ab6046, RRID: AB_2210370 Total γ-tubulin Cell Signaling Technology Cat# 5886, RRID: AB_10836184 βI-tubulin Abcam Cat# ab179511 βII-tubulin Abcam Cat# ab179512 βIII-tubulin Abcam Cat# ab18207, RRID: AB_444319 βIV-tubulin Abcam Cat# ab11315, RRID: AB_297919 Tyrosinated α-tubulin MerckMillipore Cat# ABT171 Detyrosinated α-tubulin MerckMillipore Cat# AB3201; RRID: AB_177350 Δ2 α-tubulin MerckMillipore Cat# AB3202; RRID: AB_177351 Acetyl K40 α-tubulin Abcam Cat# ab179484; Phospho S172 βIII-tubulin Abcam Cat# ab76286; RRID: AB_1523210 Polyglutaminated αβ-tubulin Adipogen Cat# AG-20B-0020; RRID: AB_2490210 SOX2 R&D Systems Cat# AF2018; RRID: AB_355110 GAPDH Cell Signaling Technology Cat# 97166 Nestin R&D Systems Cat# MAB1259; AB_2251304 Rabbit IgG, HRP-linked Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Mouse IgG, HRP-linked Cell Signaling Technology Cat# 7076; RRID: AB_330924 Mouse IgG, Alexa Fluor 488 Life Technologies Cat# A10680; RRID: AB_2534062 Rabbit IgG, AlexaFluor594 Life Technologies Cat# A11012; RRID: AB_141359 FITC-conjugated CD243 (MDR1) ThermoFisher Scientific Cat# 11-2439-41, RRID: AB_11220073 PE-eFluor610-conjugated p-Erk1/2 ThermoFisher Scientific Cat# 61-109-41 APC-conjugated p-p38 MAPK ThermoFisher Scientific Cat# 17-9078-42; RRID: AB_2573290 Anti-Rabbit Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-001 Table S3.

    Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms.
    Article Snippet: In brief Lin et al. demonstrate that PAI-1suppressed genes DKK1, SPARC, and TIMP2 exhibit increased promoter methylation in obesity-driven endometrial cancer.. This suppression event induces b-catenin uncoupling from transmembrane integrins, which triggers inside-out signaling.. Consequently, integrin engagement with epithelialdeposited ECM leads to extensive COL1 fibril branching conducive to neoplastic transformation.

    Article Title: A stress-reduced passaging technique improves the viability of human pluripotent cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ammonium bicarbonate WAKO Cat# 018-21742 1M Tris-HCl (pH 9.0) Nippon gene Cat# 314-90381 Protease Inhibitor Sigma-Aldrich Cat# P8340 Phosphatase Inhibitor cocktail 2 Sigma-Aldrich Cat# P5726 Phosphatase Inhibitor cocktail 3 Sigma-Aldrich Cat# P0044 Aurora column (25 cm length, 75 mm i.d.)

    Article Title: A stress-reduced passaging technique improves the viability of human pluripotent cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sodium pyruvate solution Sigma-Aldrich Cat# S8636 2-mercaptoethanol Thermo Fisher Scientific Cat# 21985023 Critical commercial assays Human Stem Cell Nucleofector Kit 1 Lonza Cat# VAPH-5012 Hoechst 33342 DOJINDO Cat# H342 Calcein-AM DOJINDO Cat# C396 Propidium Iodide DOJINDO Cat# P378 BCIP-NBT Solution Nacalai tesque Cat# 19880-84 0.4% Trypan blue solution WAKO Cat# 207-17081 Fixation buffer BioLegend Cat# 420801 4% Paraformaldehyde Solution Nacalai tesque Cat# 09154-85 10% TritonX-100 solution Teknova Cat# T1105 Normal donkey serum Sigma-Aldrich Cat# D9663 QIAzol lysis reagent QIAGEN Cat# 79306 miRNeasy Mini Kit QIAGEN Cat# 217004 RNase-Free DNase set QIAGEN Cat# 79254 DNeasy Blood & Tissue Kit QIAGEN Cat# 69504 ReverTra Ace qPCR RT Kit TOYOBO Cat# FSQ-101 THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat# QPX-201 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440040 TaqMan Assay, human RNase P Thermo Fisher Scientific Cat# 4403326 KOD One Master Mix TOYOBO Cat# KMM-101 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621 RIPA buffer Sigma-Aldrich Cat# R0278 cOmplete Mini EDTA-free Protease Inhibitor Cocktail Roche Cat# 4693159001 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 12-230 kDa Jess or Wes Separation Module, 8 x 25 capillary cartridges proteinsimple Cat# SM-W004 Anti-Rabbit Detection Module for Jess, Wes, Peggy Sue or Sally Sue proteinsimple Cat# DM-001 Agilent RNA6000 Pico Kit Agilent Cat# 5067-1513 Agilent High-Sensitivity DNA Kit Agilent Cat# 5067-4626 IDT for Illumina RNA UD Indexes Set A, Ligation Illumina Cat# 20040553 Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Cat# 20040529 NextSeq 500/550 High Output v2 Kit Illumina Cat# FC-404-2005 SDB-XC Empore disc cartridge 3M Cat# 2340 Sodium dodecyl sulfate (SDS) Nacalai Tesque Cat# 31606-75 Sodium deoxycholate (SDC) WAKO Cat# 190-08313 Sodium lauroyl sarcosinate (SLS) WAKO Cat# 192-10382 Trypsin / Lys-C Mix, Mass Spec Grade Promega Cat# V5072 Ethyl acetate WAKO Cat# 051-00356 Acetonitrile WAKO Cat# 018-19853 Acetic acid WAKO Cat# 018-20061 Formic acid WAKO Cat# 066-00461 Trifluoroacetic acid (TFA) WAKO Cat# 204-02743 Dithiothreitol (DTT) WAKO Cat# 045-08974 Iodoacetamide (IAA) WAKO Cat# 095-02151 (Continued on next page) Cell Reports Methods 2, 100155, February 28, 2022 e2

    Article Title: PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Tiplaxtinin (PAI-039) Selleckchem Cat# S7922 RAP Millipore Sigma Cat# 55-350-650UG MG132 Sigma Aldrich Cat# M7449-200UL RIPA buffer Thermo Fisher Scientific Cat# 89901 Fix I Buffer Fluidigm Cat# 201065 Critical Commercial Assays MILLIPLEX MAP Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay MilliporeSigma Cat# HADCYMAG-61K Qubit dsDNA HS Assay Kit Invitrogen Cat# Q32854 C1 Single-Cell mRNA Seq HT 10-17 mm IFC Fluidigm Cat# 101-4982 C1 Single-Cell mRNA Seq HT Reagent Kit v2 Fluidigm Cat# 101-3473 Nextera XT DNA Library Prep Kit Illumina Cat# FC-131-1096 Nextera XT Index Kit v2 Set A Illumina Cat# FC-131-2001 Nextera XT Index Kit v2 Set B Illumina Cat# FC-131-2002 Direct-zol RNA Microprep Kits Zymo Research Cat# R2062 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368814 LightCycler 480 SYBR Green I Master Roche Cat# 04887352001 12-230 kDa Wes Separation Module, 8 3 25 capillary cartridges ProteinSimple Cat# SM-W004 Anti-Rabbit Detection Module for Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-001 Anti-Mouse Detection Module for Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-002 2nd Generation Packaging System Mix Abcam Cat# LV003 Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma Aldrich Cat# DUO92101 Duolink In Situ Red Starter Kit Goat/Rabbit Sigma Aldrich Cat# DUO92105 Pierce Magnetic ChIP Kit ThermoFisher Scientific Cat# 26157 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat# 201060 Deposited Data Raw Single-cell RNA-seq data This paper GEO: GSE140130 Experimental Models: Cell Lines EME6/E7/TERT Kyo et al., 2003 N/A Adipose stromal cells Coriell Institute Cat# AG17870 Adipose stromal cells Zen-Bio Inc. Cat# ASC-F-SL Lot# ASC0051, ASC0053, ASC0060, ASC0061, ASC0065, ASC0064, Cat# ASC-F Lot# ASC032003, ASC050709B, Cat# ASC-F-D Lot# ASCD011906 Human endometrial epithelial cells (HEuEC) Lifeline Cell Technology FC-0078 Oligonucleotides SMAD4 shRNA Sigma-Aldrich Cat# TRCN0000040032 Primers for qRT-PCR primers, see Table S5 This paper N/A Primers for ChIP-qPCR primers, see Table S4 This paper N/A Recombinant DNA pLKO.1 puro Addgene Cat# 8453 Software and Algorithms ZEN blue software Zeiss Company N/A Seurat algorithm Butler et al., 2018 https://satijalab.org/seurat/ (Continued on next page) Cell Reports 33, 108253, October 13, 2020 e2 Article ll OPEN ACCESS

    Gentle:

    Article Title: EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency
    Article Snippet: P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat# V4XP-3032 TransIT-Lenti Transfection Reagent Mirus Cat# MIR6600 Lenti-X qRT-PCR Titration Kit Takara Cat# 631235 Lipofectamine RNAi Max reagent Thermo Fisher Scientific Cat# 13778150 Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit Thermo Fisher Scientific Cat# C10424 QIAzol lysis reagent QIAGEN Cat# 79306 RIPA Buffer Sigma-Aldrich Cat# R0278 Passive 5× Lysis Buffer Promega Cat# E1941 TRIZOL LS reagent Thermo Fisher Scientific Cat# 10296028 Direct-zol RNA Miniprep kit Zymo Research Cat# R2052 Direct-zol RNA Microprep kit Zymo Research Cat# R2062 ReverTra Ace qPCR RT Kit TOYOBO Cat# FSQ-101 THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat# QPX-201 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440040 NucleoSpin Blood XL Takara U0950B QIAamp DNA Blood Midi Kit QIAGEN Cat# 51185 QIAquick Gel Extraction Kit QIAGEN Cat# 28706 SbfI-HF New England Biolabs Cat# R3642 KOD One Master Mix TOYOBO Cat# KMM-101 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621 riboPOOLs for Ribo-seq (Homo sapiens) siTOOLs Biotech Cat# 042 riboPOOLs (Homo sapiens) siTOOLs Biotech Cat# 054 xGen UDI-UMI Adapters Integrated DNA Technologies Cat# 10005903 ProtoScript II New England Biolabs Cat# M0368L CircLigaseII ssDNA ligase Epicentre Cat# CL9025K Turbo DNase Thermo Fisher Scientific Cat# AM2238 RNase I Epicentre Cat# N6901K SUPERase-In Thermo Fisher Scientific Cat# AM2696 Phusion polymerase New England Biolabs Cat# M0530S Dr. .. GenTLE Precipitation Carrier Takara Cat# 9094 CleanCap Reagent AG TriLink BioTechnologies Cat# N-7113 N1-Methylpseudouridine 5'- Triphosphate Sodium Salt TCI Chemicals Cat# M3544 Anti-Rabbit Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-001 Anti-Mouse Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-002 Anti-Goat Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-006 Agilent RNA6000 Pico Kit Agilent Cat# 5067-1513 Agilent High-Sensitivity DNA Kit Agilent Cat# 5067-4626 Qubit RNA BR Assay Kit Thermo Fisher Scientific Cat# Q10211 IDT for Illumina RNA UD Indexes Set A, Ligation Illumina Cat# 20040553 Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Cat# 20040529 NextSeq 500/550 High Output v2 Kit Illumina Cat# FC-404-2005 NextSeq 1000/2000 P2 Reagents (100 cycles) v3 Illumina Cat# 20046811 Takara IVTpro mRNA Synthesis System Takara Cat# 6141 Nano-Glo Dual-Luciferase Reporter Assay System Promega Cat# N1610 Deposited Data SuperSeries of this study NCBI GEO GSE248820 Next generation sequencing (NGS) files (CRISPRi screening) NCBI GEO GSE248819 NGS files (RNA-seq) NCBI GEO GSE248702 NGS files (Ribosome Profiling) NCBI GEO GSE249191 NGS files (RNA-seq accompanied by Ribosome Profiling) NCBI GEO GSE249190 Raw images, flow cytometry, numerical data, and statistical results Dryad https://doi.org/10.5061/dryad.kkwh70sb6 .. WTB6 human iPSC line (58) RRID:CVCL_VM30 1B4 (CRISPRi Gen1B) human iPSC line (27) RRID:CVCL_VM35 TIG-120 human dermal fibroblast Kazuhiko Kaji RRID:CVCL_320 HDF1419 human dermal fibroblast Cell Applications, Inc. RRID:CVCL_DP65 293T/17 ATCC RRID:CVCL_1926 HepG2 JCRB RRID:CVCL_0027

    Ligation:

    Article Title: EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency
    Article Snippet: P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat# V4XP-3032 TransIT-Lenti Transfection Reagent Mirus Cat# MIR6600 Lenti-X qRT-PCR Titration Kit Takara Cat# 631235 Lipofectamine RNAi Max reagent Thermo Fisher Scientific Cat# 13778150 Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit Thermo Fisher Scientific Cat# C10424 QIAzol lysis reagent QIAGEN Cat# 79306 RIPA Buffer Sigma-Aldrich Cat# R0278 Passive 5× Lysis Buffer Promega Cat# E1941 TRIZOL LS reagent Thermo Fisher Scientific Cat# 10296028 Direct-zol RNA Miniprep kit Zymo Research Cat# R2052 Direct-zol RNA Microprep kit Zymo Research Cat# R2062 ReverTra Ace qPCR RT Kit TOYOBO Cat# FSQ-101 THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat# QPX-201 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440040 NucleoSpin Blood XL Takara U0950B QIAamp DNA Blood Midi Kit QIAGEN Cat# 51185 QIAquick Gel Extraction Kit QIAGEN Cat# 28706 SbfI-HF New England Biolabs Cat# R3642 KOD One Master Mix TOYOBO Cat# KMM-101 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621 riboPOOLs for Ribo-seq (Homo sapiens) siTOOLs Biotech Cat# 042 riboPOOLs (Homo sapiens) siTOOLs Biotech Cat# 054 xGen UDI-UMI Adapters Integrated DNA Technologies Cat# 10005903 ProtoScript II New England Biolabs Cat# M0368L CircLigaseII ssDNA ligase Epicentre Cat# CL9025K Turbo DNase Thermo Fisher Scientific Cat# AM2238 RNase I Epicentre Cat# N6901K SUPERase-In Thermo Fisher Scientific Cat# AM2696 Phusion polymerase New England Biolabs Cat# M0530S Dr. .. GenTLE Precipitation Carrier Takara Cat# 9094 CleanCap Reagent AG TriLink BioTechnologies Cat# N-7113 N1-Methylpseudouridine 5'- Triphosphate Sodium Salt TCI Chemicals Cat# M3544 Anti-Rabbit Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-001 Anti-Mouse Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-002 Anti-Goat Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-006 Agilent RNA6000 Pico Kit Agilent Cat# 5067-1513 Agilent High-Sensitivity DNA Kit Agilent Cat# 5067-4626 Qubit RNA BR Assay Kit Thermo Fisher Scientific Cat# Q10211 IDT for Illumina RNA UD Indexes Set A, Ligation Illumina Cat# 20040553 Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Cat# 20040529 NextSeq 500/550 High Output v2 Kit Illumina Cat# FC-404-2005 NextSeq 1000/2000 P2 Reagents (100 cycles) v3 Illumina Cat# 20046811 Takara IVTpro mRNA Synthesis System Takara Cat# 6141 Nano-Glo Dual-Luciferase Reporter Assay System Promega Cat# N1610 Deposited Data SuperSeries of this study NCBI GEO GSE248820 Next generation sequencing (NGS) files (CRISPRi screening) NCBI GEO GSE248819 NGS files (RNA-seq) NCBI GEO GSE248702 NGS files (Ribosome Profiling) NCBI GEO GSE249191 NGS files (RNA-seq accompanied by Ribosome Profiling) NCBI GEO GSE249190 Raw images, flow cytometry, numerical data, and statistical results Dryad https://doi.org/10.5061/dryad.kkwh70sb6 .. WTB6 human iPSC line (58) RRID:CVCL_VM30 1B4 (CRISPRi Gen1B) human iPSC line (27) RRID:CVCL_VM35 TIG-120 human dermal fibroblast Kazuhiko Kaji RRID:CVCL_320 HDF1419 human dermal fibroblast Cell Applications, Inc. RRID:CVCL_DP65 293T/17 ATCC RRID:CVCL_1926 HepG2 JCRB RRID:CVCL_0027

    Reporter Assay:

    Article Title: EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency
    Article Snippet: P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat# V4XP-3032 TransIT-Lenti Transfection Reagent Mirus Cat# MIR6600 Lenti-X qRT-PCR Titration Kit Takara Cat# 631235 Lipofectamine RNAi Max reagent Thermo Fisher Scientific Cat# 13778150 Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit Thermo Fisher Scientific Cat# C10424 QIAzol lysis reagent QIAGEN Cat# 79306 RIPA Buffer Sigma-Aldrich Cat# R0278 Passive 5× Lysis Buffer Promega Cat# E1941 TRIZOL LS reagent Thermo Fisher Scientific Cat# 10296028 Direct-zol RNA Miniprep kit Zymo Research Cat# R2052 Direct-zol RNA Microprep kit Zymo Research Cat# R2062 ReverTra Ace qPCR RT Kit TOYOBO Cat# FSQ-101 THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat# QPX-201 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440040 NucleoSpin Blood XL Takara U0950B QIAamp DNA Blood Midi Kit QIAGEN Cat# 51185 QIAquick Gel Extraction Kit QIAGEN Cat# 28706 SbfI-HF New England Biolabs Cat# R3642 KOD One Master Mix TOYOBO Cat# KMM-101 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621 riboPOOLs for Ribo-seq (Homo sapiens) siTOOLs Biotech Cat# 042 riboPOOLs (Homo sapiens) siTOOLs Biotech Cat# 054 xGen UDI-UMI Adapters Integrated DNA Technologies Cat# 10005903 ProtoScript II New England Biolabs Cat# M0368L CircLigaseII ssDNA ligase Epicentre Cat# CL9025K Turbo DNase Thermo Fisher Scientific Cat# AM2238 RNase I Epicentre Cat# N6901K SUPERase-In Thermo Fisher Scientific Cat# AM2696 Phusion polymerase New England Biolabs Cat# M0530S Dr. .. GenTLE Precipitation Carrier Takara Cat# 9094 CleanCap Reagent AG TriLink BioTechnologies Cat# N-7113 N1-Methylpseudouridine 5'- Triphosphate Sodium Salt TCI Chemicals Cat# M3544 Anti-Rabbit Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-001 Anti-Mouse Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-002 Anti-Goat Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-006 Agilent RNA6000 Pico Kit Agilent Cat# 5067-1513 Agilent High-Sensitivity DNA Kit Agilent Cat# 5067-4626 Qubit RNA BR Assay Kit Thermo Fisher Scientific Cat# Q10211 IDT for Illumina RNA UD Indexes Set A, Ligation Illumina Cat# 20040553 Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Cat# 20040529 NextSeq 500/550 High Output v2 Kit Illumina Cat# FC-404-2005 NextSeq 1000/2000 P2 Reagents (100 cycles) v3 Illumina Cat# 20046811 Takara IVTpro mRNA Synthesis System Takara Cat# 6141 Nano-Glo Dual-Luciferase Reporter Assay System Promega Cat# N1610 Deposited Data SuperSeries of this study NCBI GEO GSE248820 Next generation sequencing (NGS) files (CRISPRi screening) NCBI GEO GSE248819 NGS files (RNA-seq) NCBI GEO GSE248702 NGS files (Ribosome Profiling) NCBI GEO GSE249191 NGS files (RNA-seq accompanied by Ribosome Profiling) NCBI GEO GSE249190 Raw images, flow cytometry, numerical data, and statistical results Dryad https://doi.org/10.5061/dryad.kkwh70sb6 .. WTB6 human iPSC line (58) RRID:CVCL_VM30 1B4 (CRISPRi Gen1B) human iPSC line (27) RRID:CVCL_VM35 TIG-120 human dermal fibroblast Kazuhiko Kaji RRID:CVCL_320 HDF1419 human dermal fibroblast Cell Applications, Inc. RRID:CVCL_DP65 293T/17 ATCC RRID:CVCL_1926 HepG2 JCRB RRID:CVCL_0027

    Next-Generation Sequencing:

    Article Title: EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency
    Article Snippet: P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat# V4XP-3032 TransIT-Lenti Transfection Reagent Mirus Cat# MIR6600 Lenti-X qRT-PCR Titration Kit Takara Cat# 631235 Lipofectamine RNAi Max reagent Thermo Fisher Scientific Cat# 13778150 Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit Thermo Fisher Scientific Cat# C10424 QIAzol lysis reagent QIAGEN Cat# 79306 RIPA Buffer Sigma-Aldrich Cat# R0278 Passive 5× Lysis Buffer Promega Cat# E1941 TRIZOL LS reagent Thermo Fisher Scientific Cat# 10296028 Direct-zol RNA Miniprep kit Zymo Research Cat# R2052 Direct-zol RNA Microprep kit Zymo Research Cat# R2062 ReverTra Ace qPCR RT Kit TOYOBO Cat# FSQ-101 THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat# QPX-201 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440040 NucleoSpin Blood XL Takara U0950B QIAamp DNA Blood Midi Kit QIAGEN Cat# 51185 QIAquick Gel Extraction Kit QIAGEN Cat# 28706 SbfI-HF New England Biolabs Cat# R3642 KOD One Master Mix TOYOBO Cat# KMM-101 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621 riboPOOLs for Ribo-seq (Homo sapiens) siTOOLs Biotech Cat# 042 riboPOOLs (Homo sapiens) siTOOLs Biotech Cat# 054 xGen UDI-UMI Adapters Integrated DNA Technologies Cat# 10005903 ProtoScript II New England Biolabs Cat# M0368L CircLigaseII ssDNA ligase Epicentre Cat# CL9025K Turbo DNase Thermo Fisher Scientific Cat# AM2238 RNase I Epicentre Cat# N6901K SUPERase-In Thermo Fisher Scientific Cat# AM2696 Phusion polymerase New England Biolabs Cat# M0530S Dr. .. GenTLE Precipitation Carrier Takara Cat# 9094 CleanCap Reagent AG TriLink BioTechnologies Cat# N-7113 N1-Methylpseudouridine 5'- Triphosphate Sodium Salt TCI Chemicals Cat# M3544 Anti-Rabbit Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-001 Anti-Mouse Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-002 Anti-Goat Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-006 Agilent RNA6000 Pico Kit Agilent Cat# 5067-1513 Agilent High-Sensitivity DNA Kit Agilent Cat# 5067-4626 Qubit RNA BR Assay Kit Thermo Fisher Scientific Cat# Q10211 IDT for Illumina RNA UD Indexes Set A, Ligation Illumina Cat# 20040553 Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Cat# 20040529 NextSeq 500/550 High Output v2 Kit Illumina Cat# FC-404-2005 NextSeq 1000/2000 P2 Reagents (100 cycles) v3 Illumina Cat# 20046811 Takara IVTpro mRNA Synthesis System Takara Cat# 6141 Nano-Glo Dual-Luciferase Reporter Assay System Promega Cat# N1610 Deposited Data SuperSeries of this study NCBI GEO GSE248820 Next generation sequencing (NGS) files (CRISPRi screening) NCBI GEO GSE248819 NGS files (RNA-seq) NCBI GEO GSE248702 NGS files (Ribosome Profiling) NCBI GEO GSE249191 NGS files (RNA-seq accompanied by Ribosome Profiling) NCBI GEO GSE249190 Raw images, flow cytometry, numerical data, and statistical results Dryad https://doi.org/10.5061/dryad.kkwh70sb6 .. WTB6 human iPSC line (58) RRID:CVCL_VM30 1B4 (CRISPRi Gen1B) human iPSC line (27) RRID:CVCL_VM35 TIG-120 human dermal fibroblast Kazuhiko Kaji RRID:CVCL_320 HDF1419 human dermal fibroblast Cell Applications, Inc. RRID:CVCL_DP65 293T/17 ATCC RRID:CVCL_1926 HepG2 JCRB RRID:CVCL_0027

    RNA Sequencing:

    Article Title: EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency
    Article Snippet: P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat# V4XP-3032 TransIT-Lenti Transfection Reagent Mirus Cat# MIR6600 Lenti-X qRT-PCR Titration Kit Takara Cat# 631235 Lipofectamine RNAi Max reagent Thermo Fisher Scientific Cat# 13778150 Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit Thermo Fisher Scientific Cat# C10424 QIAzol lysis reagent QIAGEN Cat# 79306 RIPA Buffer Sigma-Aldrich Cat# R0278 Passive 5× Lysis Buffer Promega Cat# E1941 TRIZOL LS reagent Thermo Fisher Scientific Cat# 10296028 Direct-zol RNA Miniprep kit Zymo Research Cat# R2052 Direct-zol RNA Microprep kit Zymo Research Cat# R2062 ReverTra Ace qPCR RT Kit TOYOBO Cat# FSQ-101 THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat# QPX-201 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440040 NucleoSpin Blood XL Takara U0950B QIAamp DNA Blood Midi Kit QIAGEN Cat# 51185 QIAquick Gel Extraction Kit QIAGEN Cat# 28706 SbfI-HF New England Biolabs Cat# R3642 KOD One Master Mix TOYOBO Cat# KMM-101 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621 riboPOOLs for Ribo-seq (Homo sapiens) siTOOLs Biotech Cat# 042 riboPOOLs (Homo sapiens) siTOOLs Biotech Cat# 054 xGen UDI-UMI Adapters Integrated DNA Technologies Cat# 10005903 ProtoScript II New England Biolabs Cat# M0368L CircLigaseII ssDNA ligase Epicentre Cat# CL9025K Turbo DNase Thermo Fisher Scientific Cat# AM2238 RNase I Epicentre Cat# N6901K SUPERase-In Thermo Fisher Scientific Cat# AM2696 Phusion polymerase New England Biolabs Cat# M0530S Dr. .. GenTLE Precipitation Carrier Takara Cat# 9094 CleanCap Reagent AG TriLink BioTechnologies Cat# N-7113 N1-Methylpseudouridine 5'- Triphosphate Sodium Salt TCI Chemicals Cat# M3544 Anti-Rabbit Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-001 Anti-Mouse Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-002 Anti-Goat Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-006 Agilent RNA6000 Pico Kit Agilent Cat# 5067-1513 Agilent High-Sensitivity DNA Kit Agilent Cat# 5067-4626 Qubit RNA BR Assay Kit Thermo Fisher Scientific Cat# Q10211 IDT for Illumina RNA UD Indexes Set A, Ligation Illumina Cat# 20040553 Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Cat# 20040529 NextSeq 500/550 High Output v2 Kit Illumina Cat# FC-404-2005 NextSeq 1000/2000 P2 Reagents (100 cycles) v3 Illumina Cat# 20046811 Takara IVTpro mRNA Synthesis System Takara Cat# 6141 Nano-Glo Dual-Luciferase Reporter Assay System Promega Cat# N1610 Deposited Data SuperSeries of this study NCBI GEO GSE248820 Next generation sequencing (NGS) files (CRISPRi screening) NCBI GEO GSE248819 NGS files (RNA-seq) NCBI GEO GSE248702 NGS files (Ribosome Profiling) NCBI GEO GSE249191 NGS files (RNA-seq accompanied by Ribosome Profiling) NCBI GEO GSE249190 Raw images, flow cytometry, numerical data, and statistical results Dryad https://doi.org/10.5061/dryad.kkwh70sb6 .. WTB6 human iPSC line (58) RRID:CVCL_VM30 1B4 (CRISPRi Gen1B) human iPSC line (27) RRID:CVCL_VM35 TIG-120 human dermal fibroblast Kazuhiko Kaji RRID:CVCL_320 HDF1419 human dermal fibroblast Cell Applications, Inc. RRID:CVCL_DP65 293T/17 ATCC RRID:CVCL_1926 HepG2 JCRB RRID:CVCL_0027

    Flow Cytometry:

    Article Title: EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency
    Article Snippet: P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat# V4XP-3032 TransIT-Lenti Transfection Reagent Mirus Cat# MIR6600 Lenti-X qRT-PCR Titration Kit Takara Cat# 631235 Lipofectamine RNAi Max reagent Thermo Fisher Scientific Cat# 13778150 Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit Thermo Fisher Scientific Cat# C10424 QIAzol lysis reagent QIAGEN Cat# 79306 RIPA Buffer Sigma-Aldrich Cat# R0278 Passive 5× Lysis Buffer Promega Cat# E1941 TRIZOL LS reagent Thermo Fisher Scientific Cat# 10296028 Direct-zol RNA Miniprep kit Zymo Research Cat# R2052 Direct-zol RNA Microprep kit Zymo Research Cat# R2062 ReverTra Ace qPCR RT Kit TOYOBO Cat# FSQ-101 THUNDERBIRD Next SYBR qPCR Mix TOYOBO Cat# QPX-201 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440040 NucleoSpin Blood XL Takara U0950B QIAamp DNA Blood Midi Kit QIAGEN Cat# 51185 QIAquick Gel Extraction Kit QIAGEN Cat# 28706 SbfI-HF New England Biolabs Cat# R3642 KOD One Master Mix TOYOBO Cat# KMM-101 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621 riboPOOLs for Ribo-seq (Homo sapiens) siTOOLs Biotech Cat# 042 riboPOOLs (Homo sapiens) siTOOLs Biotech Cat# 054 xGen UDI-UMI Adapters Integrated DNA Technologies Cat# 10005903 ProtoScript II New England Biolabs Cat# M0368L CircLigaseII ssDNA ligase Epicentre Cat# CL9025K Turbo DNase Thermo Fisher Scientific Cat# AM2238 RNase I Epicentre Cat# N6901K SUPERase-In Thermo Fisher Scientific Cat# AM2696 Phusion polymerase New England Biolabs Cat# M0530S Dr. .. GenTLE Precipitation Carrier Takara Cat# 9094 CleanCap Reagent AG TriLink BioTechnologies Cat# N-7113 N1-Methylpseudouridine 5'- Triphosphate Sodium Salt TCI Chemicals Cat# M3544 Anti-Rabbit Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-001 Anti-Mouse Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-002 Anti-Goat Detection Module for Jess, Wes, Peggy Sue or Sally Sue ProteinSimple Cat# DM-006 Agilent RNA6000 Pico Kit Agilent Cat# 5067-1513 Agilent High-Sensitivity DNA Kit Agilent Cat# 5067-4626 Qubit RNA BR Assay Kit Thermo Fisher Scientific Cat# Q10211 IDT for Illumina RNA UD Indexes Set A, Ligation Illumina Cat# 20040553 Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Cat# 20040529 NextSeq 500/550 High Output v2 Kit Illumina Cat# FC-404-2005 NextSeq 1000/2000 P2 Reagents (100 cycles) v3 Illumina Cat# 20046811 Takara IVTpro mRNA Synthesis System Takara Cat# 6141 Nano-Glo Dual-Luciferase Reporter Assay System Promega Cat# N1610 Deposited Data SuperSeries of this study NCBI GEO GSE248820 Next generation sequencing (NGS) files (CRISPRi screening) NCBI GEO GSE248819 NGS files (RNA-seq) NCBI GEO GSE248702 NGS files (Ribosome Profiling) NCBI GEO GSE249191 NGS files (RNA-seq accompanied by Ribosome Profiling) NCBI GEO GSE249190 Raw images, flow cytometry, numerical data, and statistical results Dryad https://doi.org/10.5061/dryad.kkwh70sb6 .. WTB6 human iPSC line (58) RRID:CVCL_VM30 1B4 (CRISPRi Gen1B) human iPSC line (27) RRID:CVCL_VM35 TIG-120 human dermal fibroblast Kazuhiko Kaji RRID:CVCL_320 HDF1419 human dermal fibroblast Cell Applications, Inc. RRID:CVCL_DP65 293T/17 ATCC RRID:CVCL_1926 HepG2 JCRB RRID:CVCL_0027



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